Cell Signaling Technology
Akt1 (C73H10) Rabbit mAb
2938
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Browse Similar Products: Akt1 Western Blot Antibodies
Our lab studies mTOR signaling in macrophage polarization and function. We are actively interested in studying mTORC2 signaling in this process. By deleting Rictor in macrophages, we are able to tease apart the role of mTORC2. This antibody is easy to use and works well.
Western Blot
Murine macrophages (WT and Rictor KO)
1:2000 in 4% BSA/TBST
5% dry milk/TBST
1:5000 Rabbit IgG HRP in 5% dry milk/TBST
None
Invitrogen ECL Pico
We are able to detect total AKT1 levels in both WT and Rictor KO macrophages to prove that KO macrophage are only deficient in pAKT activity but not actual AKT protein.
28877476
N/A
Easy to use at good dilution
Great antibody to detect total AKT1 specific isoform.