Excellent Differentiation of Mouse and Human Tissue by IHC

December 13, 2018

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

COXIV (3E11) rAb

Catalog Number:

4850

This antibody is used for all of our IHC quantifications to delineate human from mouse tissues in our xenograft experiments. It works perfectly.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human MDA-MB-231 breast cancer bone metastases

Primary Incubation

1:250

Blocking Agent

Mouse-on-Mouse blocking kit, streptavidin/biotin

Secondary Incubation

Biotin anti-rabbit

Tertiary Incubation

Streptavidin HRP

Detection

DAB

Results Summary

Using this antibody reliably stains human but not mouse mitochondria in our IHC application.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works perfectly

The Bad

High concentration used, could be titrated

The Bottom Line

The best available reagent to identify human from mouse tissues by microscopy.

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