Non-Specific Dvl-2 Antibody

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

anti-Dvl-2 (10B5)

Catalog Number:

sc-8026

This antibody was used by conventional western blotting in multiple wnt-modulated cells. Results show non-specific bands without any presence of bands at the expected size in cells confirmed to express Dvl-2.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human MDA-MB-231 breast cancer cells

Primary Incubation

1:1000 in 5% milk in IBS-T

Blocking Agent

5% milk in IBS-T

Secondary Incubation

1:10000 in 5% milk in IBS-T

Tertiary Incubation

N/A

Detection

Licor infrared

Results Summary

No bands observed at the expected size.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nothing

The Bad

Doesn't work

The Bottom Line

Don't use this antibody

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