Brilliant Violet 421 Anti-Mouse CCR2 Antibody to Characterize Cardiac MFs

December 14, 2018

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

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Company:

BioLegend

Product Name:

Brilliant Violet 421 anti-mouse CCR2 antibody

Catalog Number:

150605

I study the immune responses within the heart during different forms of heart disease. This antibody was used to identify the different macrophage populations residing in the murine heart. Brilliant Violet 421 anti-mouse CCR2 Antibody is the best anti-mouse CCR2 antibody I've found so far.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cardiac tissue

Primary Incubation

15 minutes

Blocking Agent

anti-CD16/CD32 antibody

Secondary Incubation

30 minutes

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Cardiac CCR2-positive macrophages identified in tissue homogenate by flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is the best anti-mouse CCR2 antibody I've found so far to stain digested tissues

The Bad

None

The Bottom Line

The BV421 anti-mouse CCR2 antibody is the best antibody to identify recruited macrophages in digested tissues, such as the heart.

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