Great SSEA4 Antibody for Immunocytochemistry

Department of Laboratory Medicine
MacKay Memorial Hospital
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

SSEA4

Catalog Number:

41-4000

This antibody was used to detect SSEA4 expression in iPSCs by immunocytochemistry analysis.

Experimental Design and Results Summary

Applications

Immunocytochemistry

Sample

Induced pluripotent stem cell

Primary Incubation

1:100 dilution overnight at 4°C in blocking reagent

Blocking Agent

5% normal goat serum in 0.1% tween 20 / PBS

Secondary Incubation

1:200 dilution 1 hr at 25°C in blocking reagent (Alexa Fluor 488 dye

Tertiary Incubation

N/A

Detection

Fluorescence microscope

Results Summary

This antibody perfectly detected SSEA4 expression by ICC/IF in iPSCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

This antibody has high sensitivity and we will continue to use it.

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