Cell Signaling Technology
Phospho-β-Catenin (Ser33/37/Thr41) Antibody
9561
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention of colon cancer. The goal of this experiment is to determine good working antibodies for the purpose of blotting some of the core components of the WNT pathway. This antibody is a bit weak and has a few non-specific bands but overall works well for western blotting.
Western Blot
RKO (whole-cell extract)
1:1000 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% BSA in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL pico
This antibody detects beta-catenin when phosphorylated at Ser33/Ser37/Thr41 by GSK3beta. WNT3A treatment for 4 hours results in elevation of beta-catenin levels and a significant portion of this elevated pool is still phosphorylated at these sites by GSK3beta sites (feedback mechanism to maintain steady-state beta-catenin levels in cells).
N/A
Signal is a bit weak but can be improved by treating the cells with proteasome inhibitor.
A few non-specific bands
Works well for Western Blotting