R&D Systems
Human c-Myc Alexa Fluor® 488-conjugated Antibody
IC3696G
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We used the Human c-Myc Alexa Fluor® 488-conjugated Antibody from R&D Systems to detect changes in intracellular c-Myc in Mino cells treated with JQ-1, a known inhibitor of c-Myc. The antibody was great.
Flow Cytometry
Mino cell line
30 min. on ice
N/A
Flow
Mino cells were treated with JQ-1 at 1 and 2 µM for 24 hours. Intracellular c-Myc was detected after fix and permeabilization. Using c-Myc antibody at 10 µL per sample, c-Myc levels decreased with JQ-1 treatment, as seen by flow analysis.
Good quality antibody for flow
None
This antibody was great when used in flow application for intracellular c-Myc detection.