Santa Cruz
frizzled-3 Antibody (C-1)
sc-376105
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Browse Similar Products: FZD3 Western Blot Antibodies
I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I had performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the negative regulators identified in these screens is known to regulate a cell surface receptor relevant to hedgehog pathway. The goal of this experiment is to determine if this gene affects the overall levels of one of the WNT receptors, FZD3.
Western Blot
NIH-3T3 whole-cell extract
1:200 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% milk in TBS-T
1:10,000 dilution of donkey anti-mouse IgG+HRP; 1 hr at room temperature
None
ECL pico plus
This antibody detects a band at ~85 kDa. The predicted size is 76 kDa. Signal is weak and requires ECL pico plus and long exposure.
N/A
OK for Western Blotting
Weak signal
Weak signal with this antibody in NIH-3T3 cells. Signal-to-noise ratio can be improved by loading more protein.