Proteintech
IFT88 Antibody
13967-1-AP
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I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I have performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the positive regulators identified in my screens was Ift88. In this experiment, I had knocked out Ift88 in NIH-3T3 cells using lentiCRISPR v2 strategy. The result is a pooled knockout cell line for Ift88. Ift88 antibody from Proteintech picked up a band at ~93 kDa. The predicted size is 93 kDa.
Western Blot
NIH-3T3 whole-cell extract
1:500 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% milk in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL
This antibody from Proteintech picks up a band at 93 kDa. The band is specific as judged from the knockout samples.
N/A
Very good antibody for western blotting
Highly recommended for western blotting