Assess Migration of Cells Using p-Nitrophenyl Phosphate (PNPP)

October 26, 2018

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

New England BioLabs

Product Name:

p-Nitrophenyl Phosphate (PNPP)

Catalog Number:

P0757S

The aim of this experiment was to determine the migratory capacity of cells by detecting the protein phosphatase activity. The reagent was recommended by colleagues.

Experimental Design and Results Summary

Application

Migration Assay

Starting Material

Cells

Protocol Overview

Follow standard migration assay protocols. Cells were seeded in transwells.

Tips

Store the reagent at –20°C.

Results Summary

Migration of cells was detected using a standard spectrometer.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use and quick

The Bad

None

The Bottom Line

No optimization required, eliminates use of radioactivity

Join the discussion