Very Good Anti-Human CD20

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-human CD20 Antibody

Catalog Number:

302340

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+CD20+) in isolated PBMCs from peripheral blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Isolated PBMCs from HD peripheral blood

Primary Incubation

anti-CD20 BV510

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytometer

Results Summary

40% of CD19+CD20+ were detected within live lymphocytes CD3-CD14-CD16-.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background staining

The Bad

It could be cheaper

The Bottom Line

Very good option to stain human CD20

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