APC/Fire™ 750 Anti-Human CD15 (SSEA-1) Antibody

October 10, 2018

JvGCRC
Nottingham Trent University
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC/Fire™ 750 anti-human CD15 (SSEA-1) Antibody

Catalog Number:

323042

This antibody has been used to optimise flow cytometry-based immunophenotyping of whole blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. This antibody was used within a nine colour staining panel for MDSCs and DCs to distinguish granulocytic MDSC cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood

Primary Incubation

2.5uL APCFire750 CD15 for 30 minutes at 4oC

Blocking Agent

FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Beckman Coulter Galios, Kaluza software

Results Summary

Clear staining that allowed the distinction of granulocytic MDSC cells. Titration optimisations showed we could reduce the volume of antibody used with no detriment to the results. Prior to analysing samples erythrocytes were lysed using 1.5ml 1xPharmLyse (BD, cat#555899) at room temperature for 30 minutes. This antibody fit well into a nine colour panel with no compensation issues.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Clear staining and useful for FACS analysis. Tritrations showed it was possible to reduce the volume suggested by the manufacturer.

The Bad

The antibody has only been tested for flow cytometry

The Bottom Line

Easy to use antibody pack that provided clear and distinct staining of CD15+ cells

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