Biolegend
PE anti-mouse CD107a (LAMP-1) Antibody
121611
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: CD107a Flow Cytometry Antibodies
I use this antibody in a functional assay for determining NK cell degranulation. The antibody works well and gives a very strong and bright signal. The only drawback is that there is some background, but it is easily distinguished from the positive staining.
Flow Cytometry
Mouse splenic NK cells
1:100 4h at 37C
anti-mouse CD16/32
None
Flow cytomteter
Cells are stimulated with an activating antibody or target cells, and incubated with the CD107a antibody. Cells are then harvested and stained for other surface or intracellular markers, and run on the flow cytometer. This antibody detects NK cells that have degranulated upon stimulation and exposed CD107a on the cell surface.
N/A
Works well in this assay
Some high background staining
Overall a very good antibody that yields great results.