Zymo Research
ChIP DNA Clean & Concentrator Kits
D5205 and D5201
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My project focuses on the analysis of chromatin accessibility of HIV-infected primary CD4 T cells. We used a very recently developed method called ATAC-SEQ to approach this analysis. We used this kit to purify and cleanup the DNA sample after Tn5 transposase tagmentation.
DNA purification
Sample from enzymatic reaction
The kit was very easy to use: 1. mix DNA with DNA binding buffer 2. centrifuge 3. wash twice 4. elution
1. make sure you add 24ml of the 100% ethanol before use 2. the speed is by "g" not"rpm"
I used this for ATAC-SEQ protocol, the purity and quality were very good.I have used both D5205 and D5201 in my hands. the only difference is one capped and the other does not. the capped one can avoid possible contamination but each step takes more time to open and close the cap. I attached several pictures.
N/A
Very fast and with good quality
For D2501, if the sample was dropped, then you lose it
Overall, the purification kit is really good. Easy protocol, fast and short time to get your DNA with good purity.