Ebioscience
Fixable Viability Dye APC e780
65086518
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In our lab we extract single cells from mouse tissues and the many processing steps cause abundant cell death. In order to have accurate flow results, we need to stain for the dead cells and eliminate them from further analysis. This stain is very bright and allows us to easily gate out the dead cells in our samples.
Flow Cytometry
Mouse cells
Cells were stained in 100uL FACS buffer with antibody at 1:1000; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
none
LSR II
Using this dye we are easily able to identify live and dead cell populations in our samples.
N/A
Stable and bright
This is a very bright dye.