Great GFP Antibody for IHC in the Drosophila Eye

Institute of Biotechnology
National Tsing Hua University
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Genetex

Product Name:

GFP antibody

Catalog Number:

GTX13970

We express split-GFP 1-10 beta sheets in fly eye and used this antibody to detect the signals of GFP. IHC results are clear with little background.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Drosophila Eye

Primary Incubation

1:100 at 4 degree over nights

Blocking Agent

0.3%PBST with 5% NGS

Secondary Incubation

1:500 at RT for 4 hrs

Tertiary Incubation

N/A

Detection

Alexa 647

Results Summary

We express GFP in R8 photoreceptors of Drosophila and use this antibody to detect the signals of GFP. It's clear with little background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

This antibody is good to use and the host is chicken so it can avoid species crosstalk problem.

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