CD56 FITC Monoclonal Antibody

August 30, 2018

Division of BioMedical Sciences, Faculty of Medicine
Memorial University of Newfoundland
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific - Invitrogen

Product Name:

CD56 Monoclonal Antibody (MEM-188), FITC

Catalog Number:

MA1-19574

CD56 is a general marker for natural killer (NK) cells and is also expressed on a subset of CD3+ T cells. One of the focuses of our lab is studying the NK cell and NK-T cell response in the context of viral infection. In summary - a good antibody for identification of CD56+ cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC isolated from whole blood by Ficoll

Primary Incubation

FITC-conjugated anti-human CD56 antibody, incubated with approximately 1x10^6 cells for 1 hour on ice at 1:5 dilution (20ul) in a total volume of 100ul PBS+2% FCS, 1mM EDTA, 0.1% sodium azide.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD FACSCalibur

Results Summary

CD56+ cells were relatively easily distinguished from CD56- cells; however, they were not that bright. Other antibodies attached to brighter fluorophores allow clear resolution of CD56+bright and CD56+dim NK cell populations. It is difficult to identify these populations with this antibody.

DOI or PMID #

N/A

Additional Notes

Lymphocyte population was gated on for analysis

Related Categories

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Summary

The Good

Comes titrated and ready to use for minimal optimization

The Bad

Expensive, staining could be brighter.

The Bottom Line

A good antibody for identification of CD56+ cells. If you are interested in distinguishing between CD56+bright and dim populations, use a different clone directly conjugated with a brighter fluorophore.

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