Great CD185 Antibody

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

CD185 (CXCR5) Antibody from BioLegend

Product Name:

BioLegend

Catalog Number:

145520

Our focus of this project was to characterize the germinal center in a B cell-specific CD83 conditional knockout model as CD83 modulates B cell activation and germinal center response. This antibody was used to study the CD185-expression in germ center of murine spleen by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine spleen

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

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Summary

The Good

Specific signals, easy to use!

The Bad

Some background

The Bottom Line

Will buy and use it again. Recommendable!

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