Great Myc Tag Antibody for Western Blot

August 13, 2018

Institute of Biomedical Sciences
Academia Sinica
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Genetex

Product Name:

Myc tag antibody

Catalog Number:

GTX628259

In my study, I need to investigate which classical histone deacetylases (HDACs) mediate deacetylation of HO-1 in vitro. This antibody was used to detect Myc-tagged HDACs overexpression in cells by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Myc tagged protein overexpression in 293T cell line (WCL)

Primary Incubation

1:3000 dilution overnight at 4 degree in blocking agent

Blocking Agent

5% non-fat dry milk in TBST

Secondary Incubation

1:2000 dilution 1hour at room tempture in blocking agent

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody is prefectly detected Myc tagged protein by Western Blot in 293T cell line

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensivity

The Bad

N/A

The Bottom Line

We will continue to use this antibody

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