P62/SQSTM1 Antibody for Western Blot

Infectious Diseases and Vaccinology
National Health Research Institutes
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

SQSTM1 / P62 antibody

Catalog Number:

GTX629890

We wanted to detect whether P62 can be observed after Patulin treatment. This antibody was used to detect P62 expression in Raw264.7 cells by western blot analysis

Experimental Design and Results Summary

Applications

Western Blot

Sample

Raw264.7 cells whole cell lysate

Primary Incubation

1:1000 dilution 1hr at room temperature

Blocking Agent

WB: 5% non-fat dry milk in TBST

Secondary Incubation

1:5000 dilution 1hr at room temperature

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

P62 can be obviously detected after Patulin treated

DOI or PMID #

10.1074/jbc.M115.686683

Additional Notes

N/A

Related Categories

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Summary

The Good

High sensitivity

The Bad

No obvious drawback

The Bottom Line

Nice

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