Great Antibody to Detect CD90.1 Positive Mouse T Cells

July 10, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202535

Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. In order to study this process, we perform a lot of adoptive transfer experiments of donor transgenic T cells (CD90.1) into different WT B6 congenic hosts (CD90.2). In order to identify the donor T cells, we use CD90.1 to distinguish host versus donor. This marker is quite reliable since it separates quite well from the host. The BV510 Thy1.1 antibody fits nicely into certain panels since it's usually an unused channel compared to other brighter channels; however, this antibody is quite bright. We were able to readily identify the donor cells using this great and bright antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:1000, 30 minutes, 4 deg in 2% FCS/PBS

Blocking Agent

FC block cells before surface panel staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer

Results Summary

We were able to readily identify the donor cells from the host cells. Further analysis of the donor cells was done after first identifying them.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very robust and bright antibody to identify donor cells.

The Bad

Please make sure to titrate the antibody before use since it can be quite bright depending on instrument and number of cells.

The Bottom Line

Truly a great antibody to fit into many multi-parameter flow panels. We use a 12-color BD celesta (BVR), so it fits in quite nice. Since BV510 is usually on the dimmer side, this antibody fits nicely into the panel to look at more important markers from the donor cells. Also, the dilution of 1:1000 makes the antibody a cost effective purchase compared to other antibodies.

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