Adiponectin Antibody for Western Blot

General Medicine
National Taiwan University Hospital
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

National Taiwan University Hospital

Product Name:

Adiponectin antibody

Catalog Number:

GTX112777

For our research, we have isolated about 50 genes differentially expressed during adipocyte differentiation, and we have characterized several of the genes which were involved in insulin sensitivity, and clinical disorders such as obesity, type 2 diabetes and metabolic syndrome. This antibody was used to detect adiponectin expression in 3T3-L1 cell by western blot analysis. The band shown on western blot is at the right size and the intensity is strong enough.

Experimental Design and Results Summary

Applications

Western Blot

Sample

3T3-L1 cell line lysate

Primary Incubation

1:1000 dilution overnight at 4 degree in blocking reagent

Blocking Agent

5% non fat dry milk in TBST

Secondary Incubation

1:1000 dilution overnight at 4 degree in blocking reagent

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected adiponectin expression by WB in 3T3-L1 cell line.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this antibody

Share your experience with other scientists. Write a Review! »

Join the discussion