Great Cyclophilin A Antibody for Western Blot

July 12, 2018

Institute of Bioinformatics and Structural Biology & Department of Medical Sciences
National Tsing Hua University
Ph.D. Student

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Quality of Results

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Company:

Genetex

Product Name:

Cyclophilin A

Catalog Number:

GTX104698

My study focuses on oral cancer metastasis, and we are going to investigate the role of PPIA (CyclophilinA) on oral cancer metastasis. This antibody is used to detect expression in oral cancer cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Oral cancer cell line whole cell lysate

Primary Incubation

1:2000 dilution overnight at 4 degree in 1X TBST

Blocking Agent

WB: 5% non-fat dry milk in TBST

Secondary Incubation

1:10000 dilution for 1 hour at RT

Tertiary Incubation

N/A

Detection

HRP Substrate

Results Summary

This antibody perfectly detected Cyclophilin A expression by western blot

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this product.

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