Identification of Long Term HSCs With CD41

May 24, 2018

Pharmaceutical Technology
CIBER BBN
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Company:

Biolegend

Product Name:

FITC anti-mouse CD41 Antibody

Catalog Number:

133903

We use this antibody in the identification and isolation by flow cytometry of long term hematopoietic stem cells. These cells have been described as negative for this marker and are identified in combination with CD48 and CD150.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow

Primary Incubation

1:100

Blocking Agent

anti-CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

FITC channel

Results Summary

With this antibody in combination with CD150 and CD41, we analyze and sort by flow cytometry long term HSCs identified as CD150 + CD48- CD41- from gated Sca+cKit+ lineage -murine alive bone marrow cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good results

The Bad

None

The Bottom Line

Good price and performance

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