Thermo Fisher Scientific
Pierce Protease Inhibitor Mini Tablets
A32953
My goal was to analyze the expression of a number of secreted proteins in the brains of mice. Therefore this protease inhibitor was selected to be added to the homogenization buffer to reduce the proteases and thus protect the proteins during the extraction process
Protein Extraction
Mouse Brain Cells
I followed the manufacturers protocol and added 1 mini tablet to 10 ml of PBS. 1 ml of the mixture was then used to homogenize the corpus callosum.
Dissolve the tablet quickly by vortexing
The protease inhibitor worked well at maintaining the integrity of the proteins as the protein yield was high
N/A
There is no guess work. Just one tablet dissolved in 10mL.
It is a bit expensive if conducting large experiments
If you're looking for a good inhibitor cocktail this one does the job.