CD144 (VE-cadherin) Antibody

April 26, 2018

Pathology
University of California, San Francisco
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) Antibody

Catalog Number:

138006

I was attempting to use this antibody for FACS analysis to validate an animal model. I performed a titration experiment using digested murine lung tissue, which I did side by side with a CD31 antibody (worked). This antibody did not show good separation at any of the 3 concentrations I used, and I would not recommend it.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary murine lung cells

Primary Incubation

0.5 ug, 0.35 ug, and 2.5 ug per test

Blocking Agent

FC blocker (MACS)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS analysis

Results Summary

No effective seperation

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

None

The Bad

Did not work! used pretty high concentrations

The Bottom Line

Would not recommend.

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