CD11c Antibody to Detect Alveolar Macrophages

Microbiology & Immunology
University of Rochester Medical Center
Research Scientist

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Company:

Biolegend

Product Name:

Anti-mouse CD11c antibody, alexa fluor 488-conjugated

Catalog Number:

117313

Alveolar macrophages are the major immune cell type found in lung and plays crucial roles in protecting the host. Therefore, reliably detecting alveolar macrophage population from branchioalveolar lavage, lung homogenates or tissue sections is critical to successfully perform researches for respiratory diseases. To define tissue macrophages is usually not easy, so a combination of multiple antibodies is required for flow cytometry. I report a product review of detecting mouse alveolar macrophage using only two antibodies including anti-CD11c antibody from Biolegend in flow cytometry.  

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung

Primary Incubation

100 ng of antibody in 100 ul of lung homogenates resuspended in 5% FBS-containing PBS buffer

Blocking Agent

5% FBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Lung homogenate from influenza virus-infected mouse was incubated with 100 ng of anti-mouse CD11c antibody conjugated with alexa fluor 488 and 100 ng of anti-mouse SiglecF (APC-Cy7 conjugated) for 30 min at 4C. The stained cells were analyzed using LSR flow cytometer (BD). In the attached figure, alveolar macrophages in red circle were detected as CD11c (high) SiglecF (+).

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliably stains mouse alveolar macrophage

The Bad

Nothing

The Bottom Line

Mouse alveolar macrophage expresses SiglecF and high level of CD11c on the cell surface, which are useful in defining this cell population. The anti-mouse CD11c (clone: N418) conjugated with alexa fluor 488 from Biolegend reliably detects mouse alveolar macrophage population.

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