The Antibody Stains NK1.1+ Cells Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Brilliant Violet 785™ anti-mouse NK-1.1 Antibody

Catalog Number:

108749

We wanted to look at how NK cells were changing in the diseased vs healthy state, so we used this antibody to identify NK cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse colon cells

Primary Incubation

1:100 in flow staining buffer

Blocking Agent

1:100 FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

NK1.1+ cells were clearly stained with this antibody

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains NK1.1+ cells well

The Bad

Expression of NK1.1 may be pretty low, so it may be difficult to separate sometimes

The Bottom Line

The antibody identifies NK cells clearly and reliably

Share your experience with other scientists. Write a Review! »

Join the discussion