Antibody Stains Interferon gamma Producing T Cells Very Well

April 18, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse IFN-γ Antibody

Catalog Number:

505810

We wanted to look at whether activation differs between diseased and healthy mice, so we used this antibody to look at interferon gamma secretion from T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse colon cells

Primary Incubation

1:100 in flow staining buffer

Blocking Agent

1:100 FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody stains interferon-gamma-producing T cells very clearly after stimulation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Antibody clearly stains interferon-gamma-producing cells well

The Bad

None to mention

The Bottom Line

The antibody is great for assessing T cells that are producing interferon gamma

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