CD107a (LAMP-1) Antibody

April 06, 2018

Vet. pathobiology
University of Missouri
scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 594 anti-mouse CD107a (LAMP-1) Antibody

Catalog Number:

121622

Lamp-1 is located on the luminal side of lysosomes. Upon activation, Lamp-1 is transferred to the cell membrane surface of activated phagocytes. We used cells from Lamp-deficient mice to analyzed the role of phagocytosis during bacteria pathogen infection.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Bone marrow-derived macrophages from mice

Primary Incubation

1 hour

Blocking Agent

Fc blok in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flourescent Microscopy

Results Summary

Our results indicates bone marrow-derived macrophages from Lamp-1 deficient mice, unable to efficiently killing pathogenic bacteria compare to the control cells. Infected cells contains significant higher number of bacteria than the macrophages from control mice.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy performance

The Bad

N/A

The Bottom Line

Choose a better mounting buffer

Share your experience with other scientists. Write a Review! »

Join the discussion