Abcam
anti-Advillin antibody
ab7221
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Browse Similar Products: Advillin Immunofluorescence Antibodies
The goal of the experiment was to detect sensory neurons (primary afferent fibers) in the dorsal horn of the spinal cord. This is a good antibody.
Immunofluorescence
Frozen mouse sections
2 hours
Normal Donkey Serum 10%
Donkey-anti-Mouse alexa-488 conjugated Ab
None
Leica confocal microscope
Frozen section of mouse spinal cord was rinsed, permeabilized with 1% Triton for 1 hour, blocked in 10% normal donkey serum and stained with mouse anti-advillin antibody (Abcam ab72210 dilution 1/100) for 2 hours. Secondary donkey-anti-mouse Alexa 488 conjugated antibody was applied for 1 hour. Cells were rinsed and counterstained with DAPI. Results show that upper lamina of the dorsal horn (with a lot of primary afferent terminations) has positive staining. This is a good antibody.
N/A
Signal in primary afferent (where it should be)
Some background is present
Good antibody overall