Perfect Anti-VAChT Antibody

Anesthesiology
University of Wisconsin
Scientist

Overall

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Company:

MilliporeSigma

Product Name:

anti-VAChT antibody

Catalog Number:

ABN100

The goal of experiment was to detect with antibody transfected VAChT in cultured cells. Results shows perfect colocalization. This is a good antibody.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

NSC34 cells

Primary Incubation

2 hours

Blocking Agent

Normal Donkey Serum 10%

Secondary Incubation

Donkey-anti-Goat alexa 488 conjugated

Tertiary Incubation

DAPI staining

Detection

Leica confocal microscope

Results Summary

NSC34 cells were transfected with VAChT-mCherry, fixed, permeabilized with 1% Triton for 20 min, blocked in 10% normal donkey serum and stained with mouse anti-advillin antibody (Chemicon #ABN100) for 2 hours. Secondary donkey-anti-goat Cy2 conjugated antibody were applied for 1 hour. Cells were rinsed and counterstained with DAPI. Results shows perfect colocalization. This is a good antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very specific

The Bad

None

The Bottom Line

Excellent anti-VAChT antibody

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