Great Anti-Mouse CD11b PerCP-Cy5.5 Antibody

Medicine
University of Colorado Denver
Professional Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD11b Monoclonal Antibody (M1/70), PerCP-Cyanine5.5, eBioscience™

Catalog Number:

45-0112-82

We used this antibody along with Ly6G to identify neutrophils and monocytes in murine bronchoalveolar lavage. We used this product for years back when it was under Affymetrix/eBioscience. It is still produced by eBioscience under Invitrogen and our lab still thinks its a great product.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bronchoalveolar lavage cells

Primary Incubation

1:400 in FACS staining buffer, 30mins 4C or on ice, covered/in the dark

Blocking Agent

PBS with 1% BSA, 0.05% NaN3

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Bright staining, works well within our panel of lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, reliable staining

The Bad

Nothing

The Bottom Line

Good product from a good company (eBioscience & Invitrogen).

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