Biolegend
Anti-human PE/Cy7-TNFa antibody
502930
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Browse Similar Products: TNF alpha Flow Cytometry Antibodies
This antibody has been used in a flow cytometry based study immunophenotyping PBMC populations obtained from cancer patients. The results have not yet been published. Isolated PBMCs were incubated with 50ng/mL PMA, 1ug/mL ionomycin and 10ug/mL brefeldin A for 4 hours (control cells were incubated with brefeldin A only). Multi-colour antibody panels were designed to identify T cell populations and stain for the presence of multiple cytokines.
Flow Cytometry
Human peripheral blood mononuclear cells (PBMC) (fixed and permeabilized)
2.5uL PE/Cy7 TNFa for 30 minutes at 4°C
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
NA
Beckman Coulter Galios, Kaluza software
Clear staining that allowed the identification of the presence of TNFa. This antibody fit well into a ten colour panel with no compensation issues.
Clear staining and useful for FACS analysis.
The antibody has only been tested for flow cytometry
Easy to use antibody pack that provided clear and distinct staining.