Phospho-Src Family (Tyr416) from Cell Signaling Works Great

July 23, 2019

Biomedical Sciences
Cornell University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Src Family (Tyr416) Rabbit #2101

Catalog Number:

2101

Our experiments aimed to understand the molecular mechanism of retinoic acid-induced differentiation. Various proteins of the putative signaling complex were investigated. This experiment was an assay of endogenous levels of SFK pY416 in total lysate of human leukemia blasts.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary leukemia blasts and HL60 cells

Primary Incubation

Overnight, 4C

Blocking Agent

BSA

Secondary Incubation

HRP anti-rabbit IgG, 1h, RT

Tertiary Incubation

None

Detection

Western Blot

Results Summary

The antibody is sensitive and specific.

DOI or PMID #

Oncotarget. 2018; 9:4134-4149. https://doi.org/10.18632/oncotarget.23642

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody is sensitive and specific.

The Bad

None

The Bottom Line

Great antibody

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