Invitrogen Antibodies
KLRG1 Monoclonal Antibody (2F1), PE-Cyanine7
25-5893-82
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We are an immunology lab studying CD8 T cell memory differentiation. KLRG1 is a hallmark marker to identify terminal effector T cells. We performed adoptive transfer in vivo experiments, and harvest spleens during the acute response of infection. I used this antibody in my T cell panel to identify memory precursors along with terminal effector T cells. I chose this product since it fit well in my 12 color panel. Invitrogen Antibodies was one of the few vendors offering this marker with this particular clone and color.
Flow Cytometry
Murine T cells from spleen
1:500 (.2mg/mL Stock), 30 minutes at 4 deg or on ice
FC block cells before staining with surface panel staining
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Flow Cytometer
We harvested spleens at the acute stage of an immune response to analyze the CD8 T cell response. Gating on live activated CD8 T cells, we used KLRG1 and CD127 to identify terminal effector T cells versus memory precursors.
Really nice separation of positive versus negative KLRG1 expressing cells.
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Great antibody and color to identify terminal effector T cells based on this marker in CD8 T cells.