Mouse CXCR3 Antibody

February 22, 2018

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D systems

Product Name:

Mouse CXCR3 antibody

Catalog Number:

MAB1685-100

The aim was to study T cell trafficking. The specific goal was to examine the expression of CXCR3 chemokine receptor (mainly expressed by Th1 cells) by CD4 T cells. I selected this product because it was of high quality.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD4 T cells

Primary Incubation

1:20 (2ul of antibody in 40ul of sample)

Blocking Agent

5% rat serum

Secondary Incubation

1:40 (1ul of antibody in 40ul of sample) biotinylated anti-Rat IgG2a

Tertiary Incubation

1:100 - APC-conjugated streptavidin

Detection

APC

Results Summary

Excellent antibody for flow cytometry

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High quality

The Bad

None

The Bottom Line

This is much better than fluorochrome-conjugated antibody.

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