Siglec-8 PE for Staining Eosinophils

February 27, 2018

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human Siglec-8 Antibody

Catalog Number:

347103

PMNCs were extracted from Human whole blood and were stained to assess eosinophils- neutrophils percentage.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood

Primary Incubation

1/50 dilution in PBS+ 2% FBS for 15min at RT at dark.

Blocking Agent

FcR block (Miltenyi), 3min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

We can separate neutrophils CD16high and eosinophils Siglec8+. Other markers were used to confirm these cells were eosinophils ( CD15+, CCR3+).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, few ul are needed for staining

The Bad

Not always easy to gate

The Bottom Line

works well to stain human eosinophils

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