The Antibody Stains EPCAM+ Epithelial Cells Very Well

February 06, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Biotin anti-mouse CD326 (Ep-CAM) Antibody

Catalog Number:

118204

We wanted to examine the intestinal epithelial cells in the diseased vs healthy state, so we used this antibody to stain the intestinal epithelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse large intestine

Primary Incubation

1:100 in 1% BSA in PBS overnight

Blocking Agent

5% goat serum in 1% BSA

Secondary Incubation

1:200 in PBS for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

The antibody stains the intestinal epithelial cells very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains EPCAM+ epithelial cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying epithelial cells

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