Wet Transfer Using Trans-Blot Turbo Transfer System

The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. The specific goal in this experiment is to analyze protein levels by Western Blotting. We had purchased this product based on the short-duration requirement for protein transfer to nitrocellulose/PVDF membrane during Western Blotting process.