The Antibody Stains beta Catenin Very Well

January 16, 2018

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech Group Inc

Product Name:

Beta-Catenin Antibody: Rabbit Polyclonal

Catalog Number:

51067-2-AP

We wanted to study the Wnt/beta catenin pathway in the diseased vs healthy state, so we used this antibody to stain for beta catenin

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse small intestine

Primary Incubation

1:100 in 1% BSA in PBS overnight

Blocking Agent

5% goat serum in 1% BSA

Secondary Incubation

1:200 in PBS for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

The antibody stains beta catenin very well

DOI or PMID #

None

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Stains beta catenin very well

The Bad

None to mention

The Bottom Line

The antibody is great for examining the Wnt/beta catenin pathway in cells

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