Dispensing Liquid Samples and Reagents Used In Molecular Biology Lab

University of Birmingham
School of Biosciences
Postdoctoral Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

Finnpiette F2 (20 to 200 microliter) single channel pipette

Catalog Number:

4642080

I regularly work with preparing and dispensing liquids and reagents in molecular biology lab specifically preparing reagents and samples of whole genome sequencing experiments. Such experiments involve handling samples in liquid format less than 100 microliter volume with precision and accuracy in dispensing. Found that using ThermoFisher Finnpipette F2 (20 to 200 microlter) single channel pipette in dispensing right volumes was satisfactory without changes/variations in sample volume and concentration required ingredient in the liquid sample.

Experimental Design and Results Summary

Application

Preparation of samples and reagents in whole genome sequencing laboratory

Starting Material

Buffers and genomic DNA in 100 microliter of nuclease free water

Protocol Overview

Using ThermoFisher Finnpipette F2 (20 to 200 microliter volume), dispense 90 microliter HT1 Buffer hybridisation buffer to prepare 200 picomolar NGS library to a Eppendorf tube containing 10 microliter denatured sequencing DNA library. Mix well and load to Qubit Fluorimeter to quantify amount DNA in the sample been diluted.. These type of applications require precision dispensing pipettes which does not cause huge variation in volume being dispensed. ThermoFisher Finnpipette F2 are the best in my experience for such type of liquid handling.

Tips

None

Results Summary

Very satisfactory dispensing of correct volume of liquid samples as required without variation in the volume being dispensed. Some of the tests being compared to check right quantity of DNA (as measured by Qubit Fluorimeter) after using this pipette corresponded to volumes being dispensed.

Features Summary

Accurate and precise dispensing of required volumes of liquid samples, buffers and reagents in a whole genome sequence laboratory

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Light weight, ergonomic and fully autoclavable

The Bad

None

The Bottom Line

Recommend this equipment in whole genome sequencing laboratory and molecular biology lab.

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