PE Mouse IgG2b, K Isotype Ctrl Antibody For Flow Cytometry

October 24, 2017

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE Mouse IgG2b, k Isotype Ctrl Antibody

Catalog Number:

400312

The PE Mouse IgG2b, k Isotype Ctrl antibody was used as control (for background levels) to compare with ABCG2 expression. The control is easy to use for flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human ovarian cancer cells

Primary Incubation

Stained cells at 1 ug/200 uL of FACS buffer at 4 degrees Celsius for 45 minutes

Blocking Agent

2% BSA

Secondary Incubation

N/A (Conjugated)

Tertiary Incubation

N/A

Detection

FACSCalibur

Results Summary

Red indicates control antibody and blue indicates ABCG2 expression. ABCG2 expression is evident in these cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

N/A

The Bottom Line

Great antibody for flow cytometry and a valid control

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