Brilliant Violet CD4

Immunology
University of Washington
Senior Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

CD4 BV786

Catalog Number:

563331

My work looks at the interactions between host and viruses. For this series of experiments, I was phenotyping virally infected mouse cells from the spleen by flow cytometry. I chose this antibody because of the reliability that comes with BD Biosciences antibodies along with the wide array of colors that this CD4 clone comes in. The BV786 fit well into my planned fluorochrome stain for other markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

1 uL per roughly 1e6 cells in 100 uL total volume

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

There was a clear separation of the CD4+ and CD4- cells. Further, when paired with CD8, the CD3+ was easily separated into CD4+ and CD8+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, good separation

The Bad

BV786 conjugates are a little expensive

The Bottom Line

Quality antibody for routine use.

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