Abcam
Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
ab76316
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We wanted to confirm that our rats carrying a mutation in the Grm5 locus showed decreased expression of Grm5. We tested frontal cortex samples from wildtype and Grm5-heterozygous mutant rats. This antibody detected a band that appears to be Grm5.
Western Blot
Rat brain (frontal cortex)
1:10k 4C overnight in 5%BSA, 0.1% Tween-20, 1xPBS
5%BSA in 1x PBS
1:10k Licor 800 conjucated anti-rabbit prepared in 5%BSA, 1xPBS
N/A
Licor imager
This antibody detected a band at approximately 140kD that most likely corresponds to Grm5. A smaller band was also observed around 40kD that might be nonspecific. When the 140kD band was quantified it should the Grm5-het animals showed reduced expression of Grm5 compared to WT littermates.
This antibody detects Grm5 in the rat
Not much
The antibody appears to detect Grm5 with little non specific signal (perhaps a band at ~40kD). It works in rat tissue.