Isotype Control For Mouse IgG2aAPC Cy7 Labeled Antibodies

September 15, 2017

Pharmaceutical Technology
CIBER BBN
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Quality of Results

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Company:

Biolegend

Product Name:

APC/Cy7 Mouse IgG2a, κ Isotype Ctrl Antibody

Catalog Number:

400229

We use this antibody to discern between specific and unspecific signal from mouse IgG2a antibodies labeled with APC Cy7 in flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:100

Blocking Agent

We block with CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

In the APC Cy7 channel

Results Summary

We use this antibody to discard unspecific signal of mouse IgG2a antibodies labeled with APC Cy7.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good background control

The Bad

None

The Bottom Line

I highly recommend it.

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