Molecular Devices
SpectraMax M5 Multi-Mode Microplate Reader
SpectraMax M5
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The general aim of the experiment was to generate standard curve of Nile red dye in DCM. We wanted to see the concentration of nile red within linear limit of fluorescence so as to quantify the dye in sample of interest. We selected the instrument because of its sensitivity and accuracy. We previously have noticed that this instrument can detect very low fluorescence in the order of 1 RFU or less that too with very small volume of sample (200ul).
Take the blank reading in between of every reading.
N/A
Less samples, high sensitivity.
Not applicable
This can be used to estimate the amount of any fluorescent material in the sample based on standard curve.