Molecular Devices
SpectraMax M5 Multi-Mode Microplate Reader
SpectraMax M5
Info:Supplier Page View Product Specs View Product
The general aim of the experiment was to generate standard curve of Nile red dye in DCM. We wanted to see the concentration of nile red within linear limit of fluorescence so as to quantify the dye in sample of interest. We selected the instrument because of its sensitivity and accuracy. We previously have noticed that this instrument can detect very low fluorescence in the order of 1 RFU or less that too with very small volume of sample (200ul).
Generation of standard cruve
1mg/ml nile red in DCM
The dye used was obtained from Sigma and was of extra purity. The solvent used was also of extra purity.
Take the blank reading in between of every reading.
We observed that upto around 100 ug/ml and less the curve was linear, but above that concentration the signal starts to saturate. Hence this curve can be used to quantify the dye from unknown sample if the signal intensity falls in linear range. Otherwise it is recommended to dilute the sample accordingly to bring the signal intensity within linear range.
Can measure multiple excitation emission wavelength simultaneouly
N/A
Less samples, high sensitivity.
Not applicable
This can be used to estimate the amount of any fluorescent material in the sample based on standard curve.