The Role Of Pkm1 In Cancer Metabolism

July 31, 2017

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Anti-PKM1 antibody produced in rabbit

Catalog Number:

SAB4300663

Browse Similar Products: PKM1 Western Blot Antibodies

The goal is to elucidate mechanism of pkm1 and pkm2 in cancer metabolism and how the pkm2 splice variant is more aggressive in particular cancers.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa cell and MDA MB231 cell lysates.

Primary Incubation

1:1000 dilution, at room temperature for 2hr

Blocking Agent

Nonfat milk

Secondary Incubation

1:5000 dilution, 1 hr at room temperature

Tertiary Incubation

None

Detection

ECL solution

Results Summary

We got very god results at expected size with out any non-specificity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No non specific bands

The Bad

None

The Bottom Line

Highly recommended

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