TCR Vbeta12 Antibody to Detect Transduced T cells

July 21, 2017

Microbiology/Immunology
Loyola University Health science campus
PhD student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Beckman Coulter

Product Name:

anti-human VB12 antibody PE conjugated

Catalog Number:

IM2291

We transduce mouse T cells with a human T cell receptor specific to the tyrosinase antigen. We can identify TCR transduced T cells by VB12 expression using this antibody and flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T cells

Primary Incubation

25 minutes

Blocking Agent

None, examining T cells

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We are able to identify mouse T cells expressing the transduced, VB12+ TCR compared to untransduced mouse T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use; identifies distinct, positive populations

The Bad

Requires 20ul of antibody for good results

The Bottom Line

This is a good antibody to use to detect VB12 TCR T cells

Share your experience with other scientists. Write a Review! »

Join the discussion