BUB3 Western Blot Antibody

July 12, 2017

chemical biology
Indian institute of chemical technology
SRF

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Anti-BUB3 antibody produced in rabbit

Catalog Number:

B7811

We are studying spindle assembling checkpoints. Bub3 is one of the key protein to assembling and disassembling of proteins.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Different mitotic blocker treated human cell lines: HeLa, Hek,and A549 cell lines.

Primary Incubation

It is for 2 hrs at room temperature and the dilution is 1:1000

Blocking Agent

Skimmed milk

Secondary Incubation

For 1hr at room temperature

Tertiary Incubation

None

Detection

ECL solution

Results Summary

Very keen bands without any non-specificity.

DOI or PMID #

N/A

Additional Notes

No need

Related Categories

Image Gallery

Summary

The Good

No nonspecific bands

The Bad

Nothing

The Bottom Line

Highly recommended

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